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DNASE1 Native Protein

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications Bioactivity, Enzyme Activity
Concentration
LYOPH

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DNASE1 Native Protein Summary

Description
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.

Source: Native protein purified from Bovine Pancreas

Activity: >=2,000 Kunitz units per mg dry weight. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0
Preparation
Method
Native protein purified from Bovine Pancreas
Source
Bovine pancreas
Protein/Peptide Type
Native Protein
Gene
DNASE1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Bioactivity
  • Enzyme Activity
Theoretical MW
31.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Preservative
No Preservative
Concentration
LYOPH
Purity
>80% by SDS-PAGE and Coomassie blue staining
Reconstitution Instructions
Reconstitute in many buffers pH 4.5-9.0. The choice of buffer is usually application specific. For cell culture applications some labs do reconstitute DNAse I in a neutral balanced salt solution(ie HANKS, EARLES, PBS) 0.22 um filter.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNASE1 Native Protein

  • deoxyribonuclease IFLJ38093
  • deoxyribonuclease-1
  • DKFZp686H0155
  • DNase I
  • DNase I, lysosomal
  • DNL1FLJ44902
  • Dornase alfa
  • DRNI
  • EC 3.1.21.1
  • human urine deoxyribonuclease I

Background

DNASE1, also known as Deoxyribonuclease 1, is an endonuclease found in the zymogen granules of the nuclear envelope. Responsible for DNA fragmentation and cell death during apoptosis, DNASE1 binds to G-actin and blocks actin polymerization. Mutations in DNASE1 cause systemic lupus erythematosus (SLE), an autoimmune disease causing inflammation and tissue damage. DNASE1 is also known to treat symptoms of cystic fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol DNASE1