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Human DNASE1 ELISA Kit (Colorimetric)

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ELISA: Human DNASE1 ELISA Kit (Colorimetric) [NBP3-18213] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and cell culture supernates
Standard Curve Range
1.56-100 ng/ml
Sensitivity
0.03 ng/ml

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Human DNASE1 ELISA Kit (Colorimetric) Summary

Description
The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human DNASE1 in the sample, this Human DNASE1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human DNASE1 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Standard Curve Range
1.56-100 ng/ml
Sensitivity
0.03 ng/ml
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 10
Spike Recovery
80-118%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
DNASE1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Balance Solution
  2. Enzyme Conjugate
  3. Microtiter Plate
  4. Standards
  5. Stop Solution
  6. Substrate A
  7. Substrate B
  8. Wash Solution

Alternate Names for Human DNASE1 ELISA Kit (Colorimetric)

  • deoxyribonuclease IFLJ38093
  • deoxyribonuclease-1
  • DKFZp686H0155
  • DNase I
  • DNase I, lysosomal
  • DNL1FLJ44902
  • Dornase alfa
  • DRNI
  • EC 3.1.21.1
  • human urine deoxyribonuclease I

Background

DNASE1, also known as Deoxyribonuclease 1, is an endonuclease found in the zymogen granules of the nuclear envelope. Responsible for DNA fragmentation and cell death during apoptosis, DNASE1 binds to G-actin and blocks actin polymerization. Mutations in DNASE1 cause systemic lupus erythematosus (SLE), an autoimmune disease causing inflammation and tissue damage. DNASE1 is also known to treat symptoms of cystic fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol DNASE1