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DNAJB6 Antibody

Images

 
Western Blot: DNAJB6 Antibody [NBP1-82624] - Analysis in control (vector only transfected HEK293T lysate) and DNAJB6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: DNAJB6 Antibody [NBP1-82624] - Staining in human testis and pancreas tissues using anti-DNAJB6 antibody. Corresponding DNAJB6 RNA-seq data are presented for ...read more
Immunohistochemistry: DNAJB6 Antibody [NBP1-82624] - Immunohistochemical staining of human fallopian tube shows distinct positivity in ciliated cells.
Immunohistochemistry-Paraffin: DNAJB6 Antibody [NBP1-82624] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: DNAJB6 Antibody [NBP1-82624] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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DNAJB6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTS
Predicted Species
Mouse (93%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJB6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DNAJB6 Protein (NBP1-82624PEP)
Publications
Read Publication using NBP1-82624.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DNAJB6 Antibody

  • DJ4
  • DKFZp566D0824
  • DnaJ (Hsp40) homolog, subfamily B, member 6
  • dnaJ homolog subfamily B member 6
  • DnaJ
  • DnaJ-like 2 protein
  • FLJ42837
  • Heat shock protein J2
  • HHDJ1
  • HSJ2
  • HSJ-2
  • MGC1152
  • MRJMGC117297
  • MSJ1
  • MSJ-1

Background

DNAJB6 encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNAJB6 Antibody (NBP1-82624)(1)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol DNAJB6