![Western Blot: DMC1 Overexpression Lysate (Adult Normal) [NBP2-07532] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DMC1.](http://images.novusbio.com/fullsize/DMC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07532-img0001.jpg "Western Blot: DMC1 Overexpression Lysate (Adult Normal) [NBP2-07532] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DMC1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | DMC1 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC218311 Accession#: NM_007068 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | DMC1 |
| Dilutions |
|
| Application Notes | This product is intended for use as a positive control in Western Blot. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 37.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
![Western Blot Rad17 [p Ser645] Antibody](https://images.novusbio.com/images/Rad17-[p-Ser645]-Antibody-Western-Blot-NBP2-24457-img0001.jpg)
![Immunohistochemistry-Paraffin Rad17 [p Ser645] Antibody](https://images.novusbio.com/images/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-Paraffin-NBP2-24457-img0003.jpg)
![Western Blot Rad17 [p Ser645] Antibody](https://images.novusbio.com/images/Rad17-[p-Ser645]-Antibody-Western-Blot-NBP2-24457-img0005.jpg)
Research Areas for DMC1 Lysate (NBP2-07532)Find related products by research area.
|
|
Determining DMC1's role in Homologus Recombination The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DMC1 |
![Western Blot BRCA2 Antibody (234403) [Unconjugated]](https://images.novusbio.com/images2/BRCA2_MAB2476_Western_Blot_6033.jpg)
![Immunohistochemistry BRCA2 Antibody (234403) [Unconjugated]](https://images.novusbio.com/images2/mab2476_human-brca2-mab-clone-234403-immunohistochemistry-10520211657.jpg)
![Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.](https://images.novusbio.com/images/DMC1-Antibody-1D12-4-Western-Blot-NB100-181-img0003.jpg "Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.")