![Western Blot: DLL1 Antibody [NB600-628]](http://images.novusbio.com/fullsize/DLL1-Antibody-Western-Blot-NB600-628-img0002.jpg "Western Blot: DLL1 Antibody [NB600-628]")
| Reactivity | Mu, Hu, RtSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody was affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified DLL1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 150-175 of Mouse DLL1. (Uniprot: Q61483) |
| Specificity | The antibody detects DLL1 from mouse. Activity against Delta-1 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken. |
| Predicted Species | Human (96%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DLL1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested for use in ELISA against the immunizing peptide, western blot, and in IHC. Reactivity in other immunoassays is unknown. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibodyTissue: Mouse Embryonic Kidney. Fixation: FFPE buffered formalin 10% conc.Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker (left, right), EDTA pH 9.5 Pressure Cooker (middle).Primary antibody: 2ug/ml 1.5 hour @ room temp.Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45](http://images.novusbio.com/fullsize/DLL1-Antibody-Immunohistochemistry-NB600-628-img0008.jpg "Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibodyTissue: Mouse Embryonic Kidney. Fixation: FFPE buffered formalin 10% conc.Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker (left, right), EDTA pH 9.5 Pressure Cooker (middle).Primary antibody: 2ug/ml 1.5 hour @ room temp.Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45")
![Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibody. Tissue: Mouse embryonic kidney.Fixation: formalin fixed paraffin embedded 10% conc.Antigen retrieval: pH 9.5. Primary antibody: 2ug/ml for 1.5 hour @ room Temp.Secondary antibody: Peroxidase secondary reagent for 45 min at RT.Localization: as shown.Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.](http://images.novusbio.com/fullsize/DLL1-Antibody-Immunohistochemistry-NB600-628-img0007.jpg "Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibody. Tissue: Mouse embryonic kidney.Fixation: formalin fixed paraffin embedded 10% conc.Antigen retrieval: pH 9.5. Primary antibody: 2ug/ml for 1.5 hour @ room Temp.Secondary antibody: Peroxidase secondary reagent for 45 min at RT.Localization: as shown.Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.")
![ELISA: DLL1 Antibody [NB600-628] - ELISA results of purified DLL1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/DLL1-Antibody-ELISA-NB600-628-img0009.jpg "ELISA: DLL1 Antibody [NB600-628] - ELISA results of purified DLL1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")