![Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Western-Blot-NBP2-70569-img0002.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI6G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full-length protein expressed in 293T cell transfected with human DHFR expression vector |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DHFR |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 21.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Dihydrofolate Reductase/DHFR Antibody (NBP2-70569)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DHFR |
![Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70569-img0003.jpg "Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.")
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70569-img0006.jpg "Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.")
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Western-Blot-NBP2-70569-img0001.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.")
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70569-img0004.jpg "Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)")
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70569-img0005.jpg "Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)")
![Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-OTI6G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70569-img0007.jpg "Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (OTI6G7) - Azide and BSA Free [NBP2-70569] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.")
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![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")