![Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Western-Blot-NBP1-47727-img0014.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI6G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full-length protein expressed in 293T cell transfected with human DHFR expression vector |
| Specificity | This antibody is specific for Homo sapiens dihydrofolate reductase (DHFR). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DHFR |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 21.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Masashi Okada |
WB | Human | 06/23/2023 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Dihydrofolate Reductase/DHFR Antibody (NBP1-47727)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunocytochemistry-Immunofluorescence-NBP1-47727-img0008.jpg "Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.")
![Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunohistochemistry-Paraffin-NBP1-47727-img0012.jpg "Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.")
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dihydrofolate reductase.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Western-Blot-NBP1-47727-img0013.jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dihydrofolate reductase.")
![Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunohistochemistry-Paraffin-NBP1-47727-img0009.jpg "Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)")
![Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunohistochemistry-Paraffin-NBP1-47727-img0010.jpg "Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50)")
![Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Dihydrofolate-Reductase-DHFR-Antibody-6G7-Immunohistochemistry-Paraffin-NBP1-47727-img0011.jpg "Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.")
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Left: lysate of glioblastoma xenograft. Non-treated. Right: methotrexate-treated lysate of glioblastoma xenograft. 10 ug each protein were loaded.Antibody dilution is 1/3000 in 2% BSA TBST. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-47727_mouse-dihydrofolate-reductase-dhfr-mab-oti6g7-western-blot-67202311401..jpg "Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Left: lysate of glioblastoma xenograft. Non-treated. Right: methotrexate-treated lysate of glioblastoma xenograft. 10 ug each protein were loaded.Antibody dilution is 1/3000 in 2% BSA TBST. Image from verified customer review.")
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![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
