Dihydrofolate Reductase/DHFR Antibody (872442) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

DHFR (DiHydroFolate Reductase; also Tetrahydrofolate dehydrogenase) is a 21-23 kDa member of the dihydrofolate reductase family of enzymes. It is a ubiquitously expressed monomer, and considered to be a housekeeping gene. Housekeeping genes are those that play a role in multiple pathways, although not the same pathway(s) in all cells. DHFR participates in the reduction of dihydrofolate to tetrahydrofolate, a product that is subsequently used in the synthesis of purines and thymidylic acid that are used to generate both RNA and DNA. Within the cell, DHFR is known to exist in two pools: one contains DHFR bound to its own RNA where it acts as a transcriptional repressor, while another contains DHFR bound to NADPH. Human DHFR is 187 amino acids (aa) in length and possesses one DHFR domain (aa 4-185). Its mRNA binding motif is suggested to involve Cys6, Leu22, Glu30 and Ser118. There is one potential alternative start site found 75 aa upstream of the standard start site. Full length human DHFR (aa 1-187) shares 90% aa sequence identity with mouse DHFR.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.

Showing Publications 1 - 5 of 5.

Publications using MAB7934	Applications	Species
Asim Alam, Steven C. Smith, Sundaresan Gobalakrishnan, Mina McGinn, Vasily A. Yakovlev, Christopher S. Rabender Uncoupled nitric oxide synthase activity promotes colorectal cancer progression Frontiers in Oncology 3/14/2023 [PMID: 36998441]
Evan R. Abt, Thuc M. Le, Amanda M. Dann, Joseph R. Capri, Soumya Poddar, Vincent Lok et al. Reprogramming of nucleotide metabolism by interferon confers dependence on the replication stress response pathway in pancreatic cancer cells Cell Reports 1/11/2022 [PMID: 35021095]
K Oiwa, N Hosono, R Nishi, L Scotto, OA O'Connor, T Yamauchi Characterization of newly established Pralatrexate-resistant cell lines and the mechanisms of resistance BMC Cancer, 2021-07-31;21(1):879. 2021-07-31 [PMID: 34332580] (Western Blot, Human)	Western Blot	Human
Edward M. Germany, Nataliya Zahayko, Mason L. Huebsch, Jennifer L. Fox, Veena Prahlad, Oleh Khalimonchuk The AAA ATPase Afg1 preserves mitochondrial fidelity and cellular health by maintaining mitochondrial matrix proteostasis Journal of Cell Science 5/2/2018 [PMID: 30301782]
MA Fawal, T Jungas, A Kischel, C Audouard, JS Iacovoni, A Davy Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation Cell Rep, 2018-06-05;23(10):2864-2873.e7. 2018-06-05 [PMID: 29874574] (Western Blot, Mouse)	Western Blot	Mouse

Array MAB7934

• Dihydrofolate Reductase/DHFR Antibodies
• Dihydrofolate Reductase/DHFR Lysate
• Dihydrofolate Reductase/DHFR Proteins and Enzyme