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Desmoplakin Antibody

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Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879] - Paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879] - Paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Desmoplakin Antibody Summary

Immunogen
Peptide with sequence C-QESVKQAHLRNEH corresponding to internal region according to NP_004406.2.
Epitope
QESVKQAHLRNEH
Marker
Desmosomes Marker
Specificity
This antibody is expected to recognize reported isoform I (NP_004406.2) only.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DSP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1:100 - 1:2000
Application Notes
WB: Preliminary experiments in lysates of HEK293, HeLa and NIH3T3 gave no specific signal but low background. IHC-P: Human heart shows staining of the junctons between cardiomuscular fibres.
Publications
Read Publications using
NBP1-49879 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Desmoplakin Antibody

  • 250/210 kDa paraneoplastic pemphigus antigen
  • DCWHKTA
  • desmoplakin (DPI, DPII)
  • desmoplakin I
  • desmoplakin II
  • Desmoplakin
  • DP
  • DPI
  • DPII
  • DSP
  • KPPS2
  • PPKS2

Background

Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Desmoplakin
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NBP1-49879
Desmoplakin Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC

Publications for Desmoplakin Antibody (NBP1-49879)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Video Protocols

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Bioinformatics

Gene Symbol DSP
Entrez
Uniprot