Desmoglein-3 Antibody (DSG3/1535) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
DSG3/1535
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750

Order Details

Desmoglein-3 Antibody (DSG3/1535) [Alexa Fluor® 750] Summary

Immunogen
Recombinant human Desmoglein-3 extracellular protein fragment (Uniprot: P32926)
Localization
Cell Surface
Marker
Squamous Cell Marker
Specificity
Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This monoclonal antibody is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DSG3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Desmoglein-3 Antibody (DSG3/1535) [Alexa Fluor® 750]

  • Cadherin family member 6
  • CDHF6
  • CDHF6130-kD pemphigus vulgaris antigen
  • desmoglein 3 (pemphigus vulgaris antigen)
  • desmoglein 3
  • Desmoglein3
  • Desmoglein-3
  • DKFZp686P23184,130 kDa pemphigus vulgaris antigen
  • DSG3
  • pemphigus vulgaris antigen
  • PVA

Background

Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

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Bioinformatics

Gene Symbol DSG3