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DEPTOR/DEPDC6 Antibody

Images

 
Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85255] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-161
Immunocytochemistry/ Immunofluorescence: DEPTOR/DEPDC6 Antibody [NBP1-85255] - Staining of human cell line RPTEC TERT1 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85255] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DEPTOR/DEPDC6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSNKH
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DEPTOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DEPTOR/DEPDC6 Protein (NBP1-85255PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DEPTOR/DEPDC6 Antibody

  • DEP domain containing 6
  • DEP domain containing mTOR interacting protein
  • DEP domain containing MTOR-interacting protein
  • DEP domain-containing mTOR-interacting protein
  • DEP domain-containing protein 6
  • DEP.6
  • DEPDC6
  • DEPDC6FLJ12341
  • DEPTOR
  • DKFZp564B1778
  • FLJ12428
  • FLJ13854

Background

DEPTOR is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of bothcomplexes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DEPTOR