Novus Biologicals products are now on bio-techne.com

Sort By:

Products (6)

page of 1

DEPTOR/DEPDC6 Antibodies

We offer DEPTOR/DEPDC6 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each DEPTOR/DEPDC6 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DEPTOR/DEPDC6 Antibodies can be used in a variety of model species: Canine, Equine, Feline, Human, Mouse, Primate, Rat. Use the list below to choose the DEPTOR/DEPDC6 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DEPTOR/DEPDC6 polyclonal antibodies and browse our DEPTOR/DEPDC6 monoclonal antibody catalog.

Alternate Names for DEPTOR/DEPDC6 Antibodies

anti-DEPTOR/DEPDC6 antibody, anti-DEPTOR antibody, anti-DEP domain containing 6 antibody, anti-DEP domain containing mTOR interacting protein antibody, anti-DEP domain containing MTOR-interacting protein antibody, anti-DEP domain-containing mTOR-interacting protein antibody, anti-DEP domain-containing protein 6 antibody, anti-DEP.6 antibody, anti-DEPDC6FLJ12341 antibody, anti-DKFZp564B1778 antibody, anti-FLJ12428 antibody, anti-FLJ13854 antibody

6 Results for "deptor-depdc6" in Primary Antibodies

NBP1-49674

DEPTOR/DEPDC6 Antibody - BSA Free

(2 Reviews)

(21 Publications)

0.1 ml

NBP1-49674

Western Blot: DEPTOR/DEPDC6 Antibody - BSA Free [NBP1-49674] - PA regulates adipogenic differentiation by displacing DEPTOR from mTORC1. 3T3-L1 preadipocytes were transfected with a PPRE luciferase reporter plasmid vector, induced to differentiate for 3 h, and then luciferase activity was measured. 3T3-L1 cells were differentiated in the absence or presence of 300 uM C8-PA. Cell lysates and immunoprecipitates against raptor were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36968), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: DEPTOR/DEPDC6 Antibody - BSA Free [NBP1-49674] - Antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry: DEPTOR/DEPDC6 Antibody - BSA Free [NBP1-49674] - Staining of DEPTOR in mouse spleen using DAB with a hematoxylin counterstain.

Western Blot: DEPTOR/DEPDC6 Antibody - BSA Free [NBP1-49674] - Analysis of DEPDC6 in (A) human liver and (B) mouse liver.

<b>Genetic Strategies Validation. </b>Knockout Validated: DEPTOR/DEPDC6 Antibody - BSA Free [NBP1-49674] - Whole-body DEPTOR null mice are viable, fertile, normal in size, and do not display any gross physical abnormalities. Western blot showing the expression of DEPTOR/DEPDC6 in tissues of Deptor wild-type (wt/wt), heterozygote (Delta/wt) or knockout (Delta/Delta) mice. Akt was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28462079/) licensed under a CC-BY license.

No differences in the level of mTOR regulators in WT and S47 cells.(A) Cell lysates were extracted from two sets of WT and S47 MEFs and were analyzed by western blot for TSC2, DEPTOR, AKT, p53, Sco2 and GAPDH. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33170774), licensed under a CC-BY licence.

PA regulates adipogenic differentiation by displacing DEPTOR from mTORC1. (b) 3T3-L1 cells were differentiated in the absence or presence of 300 uM C8-PA. Cell lysates and immunoprecipitates against raptor were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36968), licensed under a CC-BY licence.

Effect of Hif1a deletion upon growth and invasion. (A) Wild-type (WT) mammary tumor epithelial cells (MTECs) grown to 80% confluence were subjected to hypoxic culture for the indicated times up to 24 hours, or cells continued to be cultured under normoxic conditions such that the time t = 0 sample was harvested on the same day as the time t = 24 hours hypoxic condition sample. High-salt enriched whole-cell extracts were resolved on 3% to 8% Tris-acetate gels and blotted onto polyvinylidene fluoride membrane, which was divided horizontally at approximately 60 kDa. The top half of the blot was used to detect HIF-1 alpha , and the lower portion was used to detect lamin (loading control) to avoid the need to strip and reprobe the blot. (B) Growth curve of WT and knockout (KO) MTECs cultured at normoxia (Nor) or hypoxia (Hyp) in growth medium supplemented with 5% fetal bovine serum (FBS) + epidermal growth factor (EGF) (left) or with 2% FBS (right). For cells grown in 5% FBS + EGF, a representative graph is shown in which the mean ± SEM of cell number per time point of quadruplicate wells per genotype/oxygen tension is plotted per time point. For cells grown in 2% FBS, the grand mean ± SEM of cell number is presented, which was calculated as an average of the mean cell number observed per replicates per time point as observed in three replicate experiments. All data were analyzed by two-way analysis of variance (ANOVA, *P < 0.05). (C) The mean fold change (FC) in invasion was normalized to the invasion index observed for WT cells cultured at normoxia (FC = 1.0). Data represent the mean FC in invasion observed in three independent experiments. All columns were compared using one-way ANOVA with a Bonferroni posttest. *P < 0.05. Image collected and cropped by CiteAb from the following open publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3087), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

DFMO decreases the HBV core-associated DNA and the HBc protein levels. (A) Determination of cytotoxicity of HepAD38 and HepG2.2.15 cells treated DFMO measured by the MTS assay. (B) HepAD38 cells were treated with DFMO (50 μM, 100 μM), and the HBV core-associated DNA was extracted 3 days later and measured by Southern blot (upper panel). The levels of HBc and HBs were measured by Western blotting, and capsids levels were determined using a Native gel assay (lower panel) as described above. (C) The levels of HBsAg in supernatant were measured by ELISA assay as described above. (D) HBV 3.5kb RNA levels were measured by real-time RT-PCR. (E) and (F) HepG2.2.15 cells treated with DFMO (50, 100 μM) for 3 days, then the levels of intracellular HBV DNA or HBsAg levels in the supernatant were detected by real-time PCR (E) or ELISA assay (F). (G) DFMO decreased the infection capacity of the HBV particles. HepAD38 cells in the absence of tetracycline were treated with DSMO or DFMO with indicated concentration for 3 days, and the HBV viral particles were then collected and added to the HepG2-NTCP cells. Five days later, the cytoplasmic viral DNA were extracted and measured by real-time PCR. RC, relaxed circular; DL, double stranded linear; SS, single stranded. Experiments were performed in triplicate, and data are represented as means ± SD. Statistical significance was determined by one-way ANOVA with the Tukey post-hoc test (*p < 0.05; ns, not significant). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32373551), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

C3–C3R interactions mediate developmentally-typical D1r elimination in vivo in males, but not females. Neutrophil inhibitor factor (NIF), a peptide that binds specifically to the CD11b subunit of C3 receptors (C3Rs), was assessed for its efficacy in reducing microglial phagocytic activity ex vivo. a In microglia isolated from whole brain, 60 ng, but not 120 ng NIF inhibited microglial phagocytosis of fluorescent beads (Table 3A). Representative images precede histograms; scale bar equals 20 µm. n = 4, with 2 replications/condition. b Microglia were isolated from whole brain and incubated with 60 ng NIF, and then immunocytochemically assessed for NIF and CD11b at 30, 60, and 120 min. Closed arrow heads indicate NIF (green) immunoreactivity. Scale bar equals 10 µm. There was no change in c NIF or (Table 3B) d CD11b (Table 3C) immunoreactivity over 2 h, suggesting that NIF was not causing the degradation of its receptor. n = 3; 2 replications/condition. To determine if developmental D1r downregulation requires C3–C3R interactions, NIF or Vehicle was microinjected in the NAc at e P30 in males and g P22 in females (both represent sex-specific ages prior to D1r downregulation), and then tissue was assessed at P38 and P30, respectively, an age at which D1rs should be developmentally downregulated. f In males, NIF-treated hemispheres exhibited significantly more (~25%) D1r immunoreactivity than within-animal vehicle-treated hemispheres (Table 3D). h In females, NIF-treated hemispheres exhibited the same D1r immunoreactivity as within-animal vehicle-treated hemispheres (Table 3E). Representative images precede histograms; scale bars equal 100 µm. n = 4/sex with counterbalanced injections. For ex vivo experiments, data were analyzed with one-way ANOVAs and Holm-Sidak’s post-hoc comparisons. For in vivo experiments, D1r data from 4–7 different sections per animal were averaged and then calculated as a percentage of within-animal vehicle control levels. Data were analyzed with 2-tailed one-sample t-tests. Histograms portray the mean ± SEM with individual data points overlaid. Significant post-hoc Holm-Sidak t-tests (a–d) and one-sample t-tests from 100 (f, h) (p < 0.05) comparisons are delineated with an asterisk. All statistics are in Table 3 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30254300), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-49674AF350
Alexa Fluor 405	NBP1-49674AF405
Alexa Fluor 488	NBP1-49674AF488
Alexa Fluor 532	NBP1-49674AF532
Alexa Fluor 594	NBP1-49674AF594
Alexa Fluor 647	NBP1-49674AF647
Alexa Fluor 700	NBP1-49674AF700
Alexa Fluor 750	NBP1-49674AF750
Allophycocyanin	NBP1-49674APC
Biotin	NBP1-49674B
DyLight 350	NBP1-49674UV
DyLight 405	NBP1-49674V
DyLight 488	NBP1-49674G
DyLight 550	NBP1-49674R
DyLight 594	NBP1-49674DL594
DyLight 650	NBP1-49674C
DyLight 680	NBP1-49674FR
DyLight 755	NBP1-49674IR
FITC	NBP1-49674F
HRP	NBP1-49674H
Janelia Fluor 549	NBP1-49674JF549
Janelia Fluor 646	NBP1-49674JF646
PE	NBP1-49674PE
PerCP	NBP1-49674PCP
mFluor Violet 450 SE	NBP1-49674MFV450
mFluor Violet 500 SE	NBP1-49674MFV500
mFluor Violet 610 SE	NBP1-49674MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-49674SS

compare

(select up to 3 products from this page to compare)

AF7255

DEPTOR/DEPDC6 Antibody

100 μg

AF7255

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DEPTOR/DEPDC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7255) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DEPTOR/DEPDC6 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

DEPTOR/DEPDC6 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human DEPTOR/DEPDC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7255) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7255-SP

compare

(select up to 3 products from this page to compare)

NBP2-27083

DEPTOR/DEPDC6 Antibody

0.1 mg

NBP2-27083

Western Blot: DEPTOR/DEPDC6 Antibody [NBP2-27083] - Analysis of DEPOR/DEPDC6 in human lung lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27083 at 3 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Reactivity:

Hu, Rt, Eq, Pm, Mu, Bv

Species Glossary

Reactivity: Hu, Rt, Eq, Pm, Mu, Bv

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6618A

Alt Catalog# IMG-6618A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP1-85256

DEPTOR/DEPDC6 Antibody

0.1 ml

NBP1-85256

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Staining of human pancreas shows low expression as expected.

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4

Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Staining of human adrenal gland shows high expression.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85256] - Staining in human adrenal gland and pancreas tissues using anti-DEPTOR antibody. Corresponding DEPTOR RNA-seq data are presented for the same tissues.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP1-85257

DEPTOR/DEPDC6 Antibody

0.1 ml

NBP1-85257

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85257] - Analysis in control (vector only transfected HEK293T lysate) and DEPTOR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: DEPTOR/DEPDC6 Antibody [NBP1-85257] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, mitochondria & cell junctions. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85257] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85257] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85257] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP1-85255

DEPTOR/DEPDC6 Antibody

0.1 ml

NBP1-85255

Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-85255] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-161

Immunocytochemistry/Immunofluorescence: DEPTOR/DEPDC6 Antibody [NBP1-85255] - Staining of human cell line RPTEC TERT1 shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: DEPTOR/DEPDC6 Antibody [NBP1-85255] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

page of 1

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

©2024 Novus Biologicals, All Rights Reserved.