DEPTOR/DEPDC6 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

DEPTOR (DEP domain-containing mTOR-interacting protein), also called DEP domain-containing protein 6 (DEPDC6), is a part of mTORC1 (MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR) and mTORC2 (MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR) complexes. DEPTOR functions as a negative regulator of the mTORC1/mTORC2 signaling pathways, leading to inhibition of activity of both kinase complexes. DEPTOR phosphorylation can weaken the interaction with MTOR within mTORC1 and mTORC2 complexes. DEPTOR has been shown to act as a tumor suppressor via mTORC1/mTORC2 blocking to inhibit protein synthesis as well as AKT's proliferation and survival related signaling. However, in certain conditions, DEPTOR behaves as an oncogene by relieving the feedback inhibition from S6K1 to PI3K, thus causing AKT activation. The stability of DEPTOR is controlled by SCFbeta-TrCP E3 ubiquitin ligase, and S6K1 and RSK1 are kinases that phosphorylate DEPTOR for facilitating its binding to betaTrCP for subsequent degradation. DEPTOR is down-regulated in a number of malignancies, however, is overexpressed in a subset of multiple myelomas harbouring cyclin D1/D3 or c-MAF/MAFB translocations.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 4 applications: B/N, ICC/IF, IHC-P, WB.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NBP1-49674	Applications	Species
Read, TA;Cisterna, BA;Skruber, K;Ahmadieh, S;Lindamood, HL;Vitriol, JA;Shi, Y;Lefebvre, AEYT;Black, JB;Butler, MT;Bear, JA;Cherezova, A;Ilatovskaya, DV;Weintraub, NL;Vitriol, EA; The actin binding protein profilin 1 is critical for mitochondria function bioRxiv : the preprint server for biology 2023-08-16 [PMID: 37609280] (WB)	WB
Tan M, Chang Y, Liu X et al. The Sag-Shoc2 axis regulates conversion of mPanINs to cystic lesions in Kras pancreatic tumor model Cell reports 2022-12-20 [PMID: 36543126] (IHC-P, Mouse) Details: Dilution used in IHC-P 1:400	IHC-P	Mouse
Colas C, Mouchiroud M, Al Dow M et al. DEPTOR loss impairs brown adipocyte development in vitro but has limited impacts in mice Molecular metabolism 2022-12-16 [PMID: 36535626] (WB, Mouse) Details: Dilution used in WB 1:1000	WB	Mouse
Ruiz-Serrano A, Boyle CN, MonnE RodrIguez JM et al. The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism International journal of molecular sciences 2022-01-28 [PMID: 35163456] (WB, Mouse)	WB	Mouse
Ruiz-Serrano A, MonnE RodrIguez JM, GUnter J Et al. OTUB1 regulates lung development, adult lung tissue homeostasis, and respiratory control FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-12-01 [PMID: 34793600] (WB, Human)	WB	Human
De Carvalho F, Munoz V, Brandao C Et Al. Taurine upregulates insulin signaling and mitochondrial metabolism in vitro, but not in adipocytes of obese women Nutrition 2021-09-03 [PMID: 34479044] (WB)	WB
Gnanapradeepan K, Basu S, Barnoud T et al. Increased mTOR activity and metabolic efficiency in mouse and human cells containing the African-centric tumor-predisposing p53 variant Pro47Ser Elife 2020-11-10 [PMID: 33170774]
George Sa, Kiss A, Obaid Sn et Al. p38delta genetic ablation protects female mice from anthracycline cardiotoxicity Am J Physiol Heart Circ Physiol 2020-08-22 [PMID: 32822209]
Kim D, Park G, Huuhtanen J et al. Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease Nat Commun 2020-05-07 [PMID: 32382059] (WB, Human)	WB	Human
Baek MO, Ahn CB, Cho HJ et al. Simulated microgravity inhibits C2C12 myogenesis via phospholipase D2-induced Akt/FOXO1 regulation Sci Rep 2019-10-17 [PMID: 31624287] (WB, Mouse)	WB	Mouse
Yao H, Tang H, Zhang Y, et al. DEPTOR inhibits cell proliferation and confers sensitivity to dopamine agonist in pituitary adenoma Cancer Lett. 2019-06-06 [PMID: 31176743] (IHC-P, WB, Rat)	IHC-P, WB	Rat
N Saito, J Araya, S Ito, K Tsubouchi, S Minagawa, H Hara, A Ito, T Nakano, Y Hosaka, A Ichikawa, T Kadota, M Yoshida, Y Fujita, H Utsumi, Y Kurita, K Kobayashi, M Hashimoto, H Wakui, T Numata, Y Kaneko, H Asano, M Odaka, T Ohtsuka, T Morikawa, K Nakayama, K Kuwano Involvement of Lamin B1 Reduction in Accelerated Cellular Senescence during Chronic Obstructive Pulmonary Disease Pathogenesis J. Immunol., 2019-01-28;0(0):. 2019-01-28 [PMID: 30692212] (B/N)	B/N
Suryawan A, Davis TA. Amino Acid- and Insulin-Induced Activation of mTORC1 in Neonatal Piglet Skeletal Muscle Involves Sestin2-GATOR2, Rag A/C-mTOR, and RHEB-mTOR Complex Formation. J Nutr 2018-06-01 [PMID: 29796625] (Bovine)		Bovine
Caron A, Mouchiroud M, Gautier N et al. Loss of hepatic DEPTOR alters the metabolic transition to fasting. Mol Metab. 2017-02-17 [PMID: 28462079] (WB, Mouse)	WB	Mouse
Song HI, Yoon MS. PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639. Sci Rep. 2016-11-22 [PMID: 27872488] (WB, Mouse)	WB	Mouse
Carbonneau M, M Gagne L, Lalonde ME et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun 2016-09-14 [PMID: 27624942] (WB)	WB
Caron A, Labbe SM, Lanfray D et al. Mediobasal hypothalamic overexpression of DEPTOR protects against high-fat diet-induced obesity. Mol Metab 2016-02-01 [PMID: 26909318] (WB)	WB
Caron A, Baraboi ED, Laplante M et al. DEP domain-containing mTOR-interacting protein in the rat brain: distribution of expression and potential implication J. Comp. Neurol. 2014-08-27 [PMID: 25159114]
Brundage ME, Tandon P, Eaves DW et al. MAF mediates crosstalk between Ras-MAPK and mTOR signaling in NF1. Oncogene. 2014-02-10 [PMID: 24509877] (ICC/IF, WB, Human) Details: Malignant peripheral nerve sheath (MPNST) mouse tumor cell lines, normal mouse Schwann cells, inducible MAF (iMAF)-transduced MPNST cells, & normal human Schwann cells from trauma victims, Xenograft mouse iMAF tumor models. WB: Figs 2b, 5f, 6d, 8a, IHC (P	ICC/IF, WB	Human
Fernandez-Saiz V, Targosz BS, Lemeer S et al. SCF(Fbxo9) and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma Nat Cell Biol 2012-12-23 [PMID: 23263282] (WB, Human)	WB	Human
Jegg AM, Ward TM, Iorns E et al. PI3K independent activation of mTORC1 as a target in lapatinib-resistant ERBB2+ breast cancer cells Breast Cancer Res Treat 2012-10-23 [PMID: 23089982] (WB, Human)	WB	Human
Show All 21 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	WB	Mouse	10/23/2019	View Summary Application Western Blot Sample Tested Adipose tissue Species Mouse Lot d-2 Comments Comments Mouse white adipose tissue was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody DEPTOR diluted 1:1000 in 5% BSA , before incubation with Anti-rabbit secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.
Enlarge	5 reviewed by: Verified Customer	WB		08/29/2014	View Summary Application Western Blot Lot A-2

Array NBP1-49674

• DEPTOR/DEPDC6 Antibodies
• DEPTOR/DEPDC6 Lysate
• DEPTOR/DEPDC6 Proteins