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DCPS Antibody (OTI4H8)

Images

 
Western Blot: DCPS Antibody (4H8) [NBP2-45650] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCPS.
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: DCPS Antibody (4H8) [NBP2-45650] - Analysis of extracts (35ug) from 9 different cell lines by using DCPS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Human endometrium tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DCPS Antibody (4H8) [NBP2-45650] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC
Clone
OTI4H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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DCPS Antibody (OTI4H8) Summary

Immunogen
Full length human recombinant protein of human DCPS (NP_054745) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DCPS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DCPS Antibody (OTI4H8)

  • DCS1
  • DCS-1
  • decapping enzyme, scavenger
  • EC 3.-
  • heat shock-like protein 1
  • HINT5
  • HINT-5homolog of C. elegans 7meGMP-directed hydrolase dcs-1
  • Hint-related 7meGMP-directed hydrolase
  • Histidine triad protein member 5
  • HSL1
  • HSPC015
  • mRNA decapping enzyme
  • scavenger mRNA-decapping enzyme DcpS

Background

Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DCPS Antibody (NBP2-45650) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DCPS