![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/4392R) - Azide and BSA Free [NBP3-08421] - Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin, HMW Recombinant Rabbit Monoclonal Antibody (KRTH/4392R).](http://images.novusbio.com/fullsize/Cytokeratin-HMW-Antibody-KRTH-4392R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08421-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/4392R) - Azide and BSA Free [NBP3-08421] - Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin, HMW Recombinant Rabbit Monoclonal Antibody (KRTH/4392R).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | KRTH/4392R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07286). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide corresponding to Cytokeratin, HMW residues within aa300-400 of Cytokeratin, HMW (Uniprot: Q01546) |
| Localization | Cytoplasmic |
| Marker | Epithelial Marker |
| Specificity | The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT76 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT76 |
![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/4392R) - Azide and BSA Free [NBP3-08421] - Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin, HMW Recombinant Rabbit Monoclonal Antibody (KRTH/4392R).](http://images.novusbio.com/fullsize/Cytokeratin-HMW-Antibody-KRTH-4392R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08421-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/4392R) - Azide and BSA Free [NBP3-08421] - Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin, HMW Recombinant Rabbit Monoclonal Antibody (KRTH/4392R).")
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