![Western Blot: Cytokeratin 8 Antibody (TS1) - Azide and BSA Free [NBP2-34501] - Western Blot Analysis of SK-OV-3 cell lysate using Cytokeratin 8 Antibody (TS1).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-TS1-Azide-and-BSA-Free-Western-Blot-NBP2-34501-img0002.jpg "Western Blot: Cytokeratin 8 Antibody (TS1) - Azide and BSA Free [NBP2-34501] - Western Blot Analysis of SK-OV-3 cell lysate using Cytokeratin 8 Antibody (TS1).")
| Reactivity | Hu, Ma(-), Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | TS1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34267). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Keratin preparation from a human carcinoma (Uniprot: P05787) |
| Epitope | aa343-357 (ELAIKDANAKLSELE) |
| Localization | Cytoplasmic |
| Specificity | Epitope of this monoclonal antibody is located between aa343-357 (ELAIKDANAKLSELE). Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT8 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 52.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 8 Antibody (NBP2-34501)Find related products by research area.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
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| Gene Symbol | KRT8 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (TS1) - Azide and BSA Free [NBP2-34501] - Immunofluorescence Analysis of HeLa cells labeling CK8 with Cytokeratin 8 Antibody (TS1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-TS1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34501-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (TS1) - Azide and BSA Free [NBP2-34501] - Immunofluorescence Analysis of HeLa cells labeling CK8 with Cytokeratin 8 Antibody (TS1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
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