![Western Blot: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytokera](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-71082-img0002.jpg "Western Blot: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytokera")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI1B12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 53.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 8 Antibody (NBP2-71082)Find related products by research area.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT8 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71082-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.")
![Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71082-img0010.jpg "Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
![Flow Cytometry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71082-img0011.jpg "Flow Cytometry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution")
![Western Blot: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 8 monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-71082-img0001.jpg "Western Blot: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 8 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of A549 cells using anti-Cytokeratin 8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71082-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of A549 cells using anti-Cytokeratin 8 mouse monoclonal antibody.")
![Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71082-img0006.jpg "Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
![Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71082-img0007.jpg "Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
![Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71082-img0008.jpg "Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
![Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71082-img0009.jpg "Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
![Flow Cytometry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of HeLa cells using anti-Cytokeratin 8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71082-img0004.jpg "Flow Cytometry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of HeLa cells using anti-Cytokeratin 8 mouse monoclonal antibody.")
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