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Cytokeratin 8/18 Antibody (C-51) [HRP]

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Product Details

Summary
Reactivity Hu, Rt, Po, Bv, Ma(-), Pm, Sh, Ch(-), Do(-), Mu(-), Rb(-), Xp(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, ICC/IF
Clone
C-51
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-11565AF350
Alexa Fluor 405
NBP3-11565AF405
Alexa Fluor 488
NBP3-11565AF488
Alexa Fluor 532
NBP3-11565AF532
Alexa Fluor 594
NBP3-11565AF594
Alexa Fluor 647
NBP3-11565AF647
Alexa Fluor 700
NBP3-11565AF700
Alexa Fluor 750
NBP3-11565AF750
Allophycocyanin
NBP3-11565APC
Biotin
NBP3-11565B
CoraFluor 1
NBP3-11565CL1
DyLight 350
NBP3-11565UV
DyLight 405
NBP3-11565V
DyLight 488
NBP3-11565G
DyLight 550
NBP3-11565R
DyLight 594
NBP3-11565DL594
DyLight 650
NBP3-11565C
DyLight 680
NBP3-11565FR
DyLight 755
NBP3-11565IR
FITC
NBP3-11565F
Janelia Fluor 525
NBP3-11565JF525
Janelia Fluor 549
NBP3-11565JF549
Janelia Fluor 646
NBP3-11565JF646
Janelia Fluor 669
NBP3-11565JF669
PE
NBP3-11565PE
PerCP
NBP3-11565PCP
Unconjugated
NBP2-44929-0.1mg
mFluor Violet 450 SE
NBP3-11565MFV450
mFluor Violet 500 SE
NBP3-11565MFV500
mFluor Violet 610 SE
NBP3-11565MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44929-0.1mg
IHC-Prediluted
NBP2-48179
Azide and BSA Free
NBP3-11565
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Cytokeratin 8/18 Antibody (C-51) [HRP] Summary

Immunogen
Cytoskeleton preparation from HeLa cells
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Mouse, Ferret, Rabbit, donkey, Chicken or Xenopus laevis.

Rat reactivity reported in scientific liteRature (PMID: 28975767).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Alternate Names for Cytokeratin 8/18 Antibody (C-51) [HRP]

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin-8
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Cytokeratin 8/18
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NBP3-11565H
Cytokeratin 8/18 Antibody (C-51) [HRP]
Species: Hu, Rt, Po, Bv, Ma(-), Pm, Sh, Ch(-), Do(-), Mu(-), Rb(-), Xp(-)
Applications: WB, Flow, ICC/IF, IHC, ICC/IF

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Bioinformatics

Gene Symbol KRT8