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Cytokeratin 8/18 Antibody (C-51) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Hu, Rt, Po, Bv, Ma(-), Pm, Sh, Ch(-), Do(-), Mu(-), Rb(-), Xp(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, ICC/IF
Clone
C-51
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594

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Cytokeratin 8/18 Antibody (C-51) [Alexa Fluor® 594] Summary

Immunogen
Cytoskeleton preparation from HeLa cells
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Mouse, Ferret, Rabbit, donkey, Chicken or Xenopus laevis.

Rat reactivity reported in scientific liteRature (PMID: 28975767).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Cytokeratin 8/18 Antibody (C-51) [Alexa Fluor® 594]

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin-8
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rt, Po, Bv, Ma(-), Pm, Sh, Ch(-), Do(-), Mu(-), Rb(-), Xp(-)
Applications: WB, Flow, ICC/IF, IHC, ICC/IF

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Cytokeratin 8/18 Products

Array NBP3-11565AF594

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Bioinformatics

Gene Symbol KRT8