![Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 10 Monoclonal Antibody (SPM623).](http://images.novusbio.com/fullsize/Cytokeratin-10-Antibody-SPM623-Immunohistochemistry-Paraffin-NBP2-53134-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 10 Monoclonal Antibody (SPM623).")
| Reactivity | Hu, Ca, FeSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | SPM623 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54332) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Cytoskeletal preparation extracted from human ectocervical epithelium (Uniprot: P13645) |
| Localization | Cytoplasmic |
| Marker | Suprabasal Epithelial Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT10 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 56.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134] - CK10-positive human FFPE skin tissue section. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-53134_mouse-monoclonal-cytokeratin-10-antibody-spm623-17720241044637.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134] - CK10-positive human FFPE skin tissue section. Image from verified customer review.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")