![Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Analysis of CYB5A expression in transfected 293T cell line by CYB5A polyclonal antibody. A: Non-transfected lysate. B: CYB5A transfected lysate. 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Cytochrome-b5-type-A-Antibody-Western-Blot-NBP2-14923-img0002.jpg "Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Analysis of CYB5A expression in transfected 293T cell line by CYB5A polyclonal antibody. A: Non-transfected lysate. B: CYB5A transfected lysate. 12% SDS PAGE, antibody diluted at 1:500.")
| Reactivity | Hu, Ca, Fe, Po, Bv, Ca, Pm, ShSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytochrome b5 type A. The exact sequence is proprietary. |
| Predicted Species | Porcine (92%), Bovine (91%), Monkey (97%), Canine (95%), Sheep (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CYB5A |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14923_rabbit-polyclonal-cytochrome-b5-type-a-antibody-910202420565.jpg "Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-14923_rabbit-polyclonal-cytochrome-b5-type-a-antibody-91020242001052.jpg "Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-14923] - Cytochrome b5 type A antibody detects Cytochrome b5 type A protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytochrome b5 type A stained by Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-14923_rabbit-polyclonal-cytochrome-b5-type-a-antibody-910202420115095.jpg "Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-14923] - Cytochrome b5 type A antibody detects Cytochrome b5 type A protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytochrome b5 type A stained by Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-14923] - Cytochrome b5 type A antibody detects Cytochrome b5 type A protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytochrome b5 type A stained by Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-14923_rabbit-polyclonal-cytochrome-b5-type-a-antibody-91020242014649.jpg "Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-14923] - Cytochrome b5 type A antibody detects Cytochrome b5 type A protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytochrome b5 type A stained by Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500.Blue: Hoechst 33342 staining.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")