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CYRPTIC/CFC1 Antibody (221428) [Unconjugated]

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Cryptic was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse Cryptic Monoclonal Antibody (Catalog # MAB18401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Cryptic was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse Cryptic Monoclonal Antibody (Catalog # MAB18401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
221428
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB18401U-100UG
Alexa Fluor 405
FAB18401V-100UG
Alexa Fluor 488
FAB18401G-100UG
Alexa Fluor 532
FAB18401X
Alexa Fluor 594
FAB18401T-100UG
Alexa Fluor 647
FAB18401R-100UG
Alexa Fluor 700
FAB18401N-100UG
Alexa Fluor 750
FAB18401S-100UG
Allophycocyanin
FAB18401A
Allophycocyanin/Cy7
FAB18401APCCY7
Biotin
FAB18401B
CoraFluor 1
FAB18401CL1
DyLight 350
FAB18401D
DyLight 405
FAB18401E
DyLight 488
FAB18401K
DyLight 550
FAB18401L
DyLight 594
FAB18401M
DyLight 650
FAB18401W
DyLight 680
FAB18401Y
DyLight 755
FAB18401Z
FITC
FAB18401F
HRP
FAB18401H
Janelia Fluor 525
FAB18401JF525
Janelia Fluor 549
FAB18401I
Janelia Fluor 646
FAB18401J
Janelia Fluor 669
FAB18401JF669
PE
FAB18401P
PE/Atto594
FAB18401PEATT594
PE/Cy5.5
FAB18401PECY55
PE/Cy7
FAB18401PECY7
PerCP
FAB18401C
mFluor Violet 450 SE
FAB18401MFV450
mFluor Violet 500 SE
FAB18401MFV500
mFluor Violet 610 SE
FAB18401MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB18401-SP
Carrier Free
FAB18401V-100UG
Carrier Free
FAB18401U-100UG
Carrier Free
FAB18401T-100UG
Carrier Free
FAB18401S-100UG
Carrier Free
FAB18401R-100UG
Carrier Free
FAB18401N-100UG
Carrier Free
FAB18401G-100UG
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CYRPTIC/CFC1 Antibody (221428) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cryptic
Tyr36-Gly177
Accession # P97766
Specificity
Detects mouse Cryptic in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human Cryptic is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CFC1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CYRPTIC/CFC1 Antibody (221428) [Unconjugated]

  • CFC1
  • CFC-1
  • CFC1B
  • cripto, FRL-1, cryptic family 1
  • Cryptic family protein 1
  • cryptic protein
  • Cryptic
  • CRYPTICFLJ77897
  • HTX2MGC133213

Background

Cryptic, also known as CFC-1, is a member of the Epidermal Growth Factor-Cripto/FRL-1/Cryptic (EGF-CFC) family of signaling proteins. It is expressed during mesoderm differentation and is necessary for determining left-right asymmetry. Cryptic functions as a co-receptor that regulates signaling by some TGF-beta superfamily members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CFC1
Uniprot