![Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-Western-Blot-NBP2-37247-img0004.jpg "Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, CyTOF-ready |
| Clone | 5F8C5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Cyclin E1 (AA: 307-410) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CCNE1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry Cyclin E2 Antibody (718916) [Unconjugated]](https://images.novusbio.com/images2/Cyclin_E2_MAB7444_Immunohistochemistry_16977.jpg)
![Immunohistochemistry Cyclin E2 Antibody (718916) [Unconjugated]](https://images.novusbio.com/images2/12177.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin E1 Antibody (NBP2-37247)Find related products by research area.
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| Gene Symbol | CCNE1 |
![Flow Cytometry: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Flow cytometric analysis of K652 cells using CCNE1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-Flow-Cytometry-NBP2-37247-img0005.jpg "Flow Cytometry: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Flow cytometric analysis of K652 cells using CCNE1 mouse mAb (green) and negative control (red).")
![Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against human CCNE1 (AA: 307-410) recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-Western-Blot-NBP2-37247-img0002.jpg "Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against human CCNE1 (AA: 307-410) recombinant protein. (Expected MW is 37.5 kDa)")
![Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against HEK293 (1) and CCNE1 (AA: 307-410)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-Western-Blot-NBP2-37247-img0003.jpg "Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against HEK293 (1) and CCNE1 (AA: 307-410)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-5F8C5-ELISA-NBP2-37247-img0001.jpg "ELISA: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
![Immunohistochemistry Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Immunohistochemistry_11792.jpg)
![Western Blot Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Western_Blot_9526.jpg)
![Simple Western Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Simple_Western_20094.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")