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CYBB/NOX2 Antibody (NL7) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
NL7
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

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CYBB/NOX2 Antibody (NL7) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Cytochrome B245 solubilized in octyl-beta-glucopyranoside
Epitope
Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.
Specificity
This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CYBB/NOX2 Antibody (NL7) [CoraFluor™ 1]

  • CGD
  • CGD91-phox
  • CYBB
  • CYBB/NOX2
  • Cytochrome b(558) subunit beta
  • cytochrome b-245 heavy chain
  • cytochrome b-245, beta polypeptide
  • Cytochrome b558 subunit beta
  • EC 1.-.-.-
  • EC 1.6.3
  • gp91-1
  • GP91PHOX
  • gp91-phox
  • Heme-binding membrane glycoprotein gp91phox
  • NADPH oxidase 2
  • Neutrophil cytochrome b 91 kDa polypeptide
  • NOX2
  • NOX2chronic granulomatous disease
  • p22 phagocyte B-cytochrome
  • p91-PHOX
  • Superoxide-generating NADPH oxidase heavy chain subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYBB/NOX2 Antibody (NBP1-41012CL1) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYBB