Home » CTDSP1 » CTDSP1 Lysates » CTDSP1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

CTDSP1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: CTDSP1 Overexpression Lysate (Adult Normal) [NBL1-09567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CTDSP1.
Western Blot: CTDSP1 Overexpression Lysate (Adult Normal) [NBL1-09567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CTDSP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CTDSP1 Overexpression Lysate Summary

Description

CTDSP1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC212037

Accession#: NM_021198

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CTDSP1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for CTDSP1 Overexpression Lysate

  • carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
  • CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) smallphosphatase 1
  • NIF3
  • NLI-IF
  • NLIIFEC 3.1.3.16
  • NLI-interacting factor 3
  • SCP1Nuclear LIM interactor-interacting factor 3
  • Small CTD phosphatase 1
  • Small C-terminal domain phosphatase 1

Background

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in theC-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase IItranscription, possibly by controlling the transition from initiation/capping to processive transcript elongation.Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronalcells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

SCP3/SYCP3
Antibody - BSA Free
NB300-232
Western Blot SCP3/SYCP3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence SCP3/SYCP3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence SCP3/SYCP3 Antibody - BSA Free
(2 Reviews)
(82 Publications)
Species: Bv, Ch, Fe, Hu, Mu, Pa, Po, Rt
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
SCP1 Antibody -
BSA Free
NB300-229
Immunocytochemistry/ Immunofluorescence SCP1 Antibody - BSA Free
Western Blot SCP1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence SCP1 Antibody - BSA Free
(3 Reviews)
(57 Publications)
Species: Ch, Hu(-), Ma, Pm, Mu, Pa, Rt
Applications: ChIP, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
CTDSPL Antibody
NBP1-20903
Immunocytochemistry/ Immunofluorescence CTDSPL Antibody
Flow Cytometry CTDSPL Antibody
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, PEP-ELISA
TCIRG1 Antibody
(6H3)
H00010312-M01
Western Blot TCIRG1 Antibody (6H3)
(5 Publications)
Species: Hu, Mu
Applications: ELISA, IHC, IHC-Fr, WB
CTDSP2 Antibody
NBP1-32283
Western Blot CTDSP2 Antibody
Western Blot CTDSP2 Antibody
Western Blot CTDSP2 Antibody
Species: Hu, Mu, Rt
Applications: WB
Sterol carrier
protein 2 Antibody
NBP1-85958
Immunocytochemistry/ Immunofluorescence Sterol carrier protein 2 Antibody
Western Blot Sterol carrier protein 2 Antibody
Western Blot Sterol carrier protein 2 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
SCP2/SYCP2
Antibody - Azide and ...
NBP2-94045
Western Blot SCP2/SYCP2 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence SCP2/SYCP2 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
FCP1 Antibody (3G4)
H00009150-M04
ELISA FCP1 Antibody (3G4)
Immunocytochemistry/ Immunofluorescence FCP1 Antibody (3G4)
Immunohistochemistry-Paraffin FCP1 Antibody (3G4)
(1 Publication)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
ERMAP Antibody
[Unconjugated]
AF4928
Flow Cytometry ERMAP Antibody [Unconjugated]
Species: Hu
Applications: CyTOF-ready, Flow, WB

Transgelin/TAGLN/SM22
NB600-507
Western Blot Transgelin/TAGLN/SM22 alpha Antibody
Immunohistochemistry-Paraffin Transgelin/TAGLN/SM22 alpha Antibody
Western Blot Transgelin/TAGLN/SM22 alpha Antibody
(1 Review)
(23 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, KD, PEP-ELISA, WB
Oct-3/4 Antibody
[Unconjugated]
AF1759
Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]
Immunocytochemistry Oct-3/4 Antibody [Unconjugated]
Simple Western Oct-3/4 Antibody [Unconjugated]
(75 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
NRAMP1/SLC11A1
Antibody (910825)
MAB8400
Western Blot NRAMP1/SLC11A1 Antibody (910825)
Flow Cytometry NRAMP1/SLC11A1 Antibody (910825)
Species: Hu
Applications: CyTOF-ready, Flow, WB
CTAG1B Antibody
(OTI2B6)
NBP1-47714
Immunocytochemistry/ Immunofluorescence CTAG1B Antibody (OTI2B6)
Flow Cytometry CTAG1B Antibody (OTI2B6)
Flow Cytometry CTAG1B Antibody (OTI2B6)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(515 Publications)
Species: Hu
Applications: BA, BA
Urocortin 3
Antibody
NB100-93444
Western Blot Urocortin 3 Antibody
(1 Publication)
Species: Hu
Applications: PEP-ELISA, WB
NRSF Antibody
NB100-757
Immunohistochemistry-Paraffin NRSF Antibody
Western Blot NRSF Antibody
(1 Review)
(1 Publication)
Species: Hu
Applications: ChIP, IHC, IHC-P, WB
GARS Antibody
NBP3-35329
GARS Antibody
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB
Recombinant Human
RAG1AP1 GST (N-Term) ...
H00055974-P01
Other Recombinant Human RAG1AP1 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, WB
GAD1/GAD67
Antibody [Unconjugate
AF2086
Immunohistochemistry GAD1/GAD67 Antibody [Unconjugated]
Western Blot GAD1/GAD67 Antibody [Unconjugated]
Simple Western GAD1/GAD67 Antibody [Unconjugated]
(13 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
Smad1 Antibody
[Unconjugated]
AF2039
Immunohistochemistry Smad1 Antibody [Unconjugated]
Western Blot Smad1 Antibody [Unconjugated]
Simple Western Smad1 Antibody [Unconjugated]
(8 Publications)
Species: Hu
Applications: IHC, Simple Western, WB

Publications for CTDSP1 Lysate (NBL1-09567) (0)

There are no publications for CTDSP1 Lysate (NBL1-09567).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CTDSP1 Lysate (NBL1-09567) (0)

There are no reviews for CTDSP1 Lysate (NBL1-09567). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CTDSP1 Lysate (NBL1-09567) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CTDSP1 Products

Research Areas for CTDSP1 Lysate (NBL1-09567)

Find related products by research area.

Blogs on CTDSP1

There are no specific blogs for CTDSP1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western Blot: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - Analysis of SCP3 in mouse testis protein.
SCP3/SYCP3 Antibody - BSA Free
NB300-232

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CTDSP1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol CTDSP1