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CPI17 alpha Antibody (4H10) - BSA Free

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Western Blot: CPI17 alpha Antibody (4H10) [NBP1-04336] - The Cell lysates of A549 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1R14A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: CPI17 alpha Antibody (4H10) [NBP1-04336] - The Cell lysates of A549 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1R14A (1:500). Proteins were visualized using ...read more
Western Blot: CPI17 alpha Antibody (4H10) [NBP1-04336] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500). Proteins were visualized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-04336AF350
Alexa Fluor 405
NBP1-04336AF405
Alexa Fluor 488
NBP1-04336AF488
Alexa Fluor 532
NBP1-04336AF532
Alexa Fluor 594
NBP1-04336AF594
Alexa Fluor 647
NBP1-04336AF647
Alexa Fluor 700
NBP1-04336AF700
Alexa Fluor 750
NBP1-04336AF750
Biotin
NBP1-04336B
CoraFluor 1
NBP1-04336CL1
DyLight 350
NBP1-04336UV
DyLight 405
NBP1-04336V
DyLight 488
NBP1-04336G
DyLight 550
NBP1-04336R
DyLight 594
NBP1-04336DL594
DyLight 650
NBP1-04336C
DyLight 680
NBP1-04336FR
DyLight 755
NBP1-04336IR
FITC
NBP1-04336F
HRP
NBP1-04336H
Janelia Fluor 525
NBP1-04336JF525
Janelia Fluor 549
NBP1-04336JF549
Janelia Fluor 585
NBP1-04336JF585
Janelia Fluor 635
NBP1-04336JF635
Janelia Fluor 646
NBP1-04336JF646
Janelia Fluor 669
NBP1-04336JF669
mFluor Violet 450 SE
NBP1-04336MFV450
mFluor Violet 500 SE
NBP1-04336MFV500
mFluor Violet 610 SE
NBP1-04336MFV610
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CPI17 alpha Antibody (4H10) - BSA Free Summary

Immunogen
Recombinant human CPI17 alpha (1-147aa) purified from E. coli
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PPP1R14A
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:250-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CPI17 alpha Antibody (4H10) - BSA Free

  • 17 kDa PKC-potentiated inhibitory protein of PP1
  • CPI-1717-kDa PKC-potentiated inhibitory protein of PP1
  • CPI1717-KDa protein
  • PKC-potentiated inhibitory protein of PP1
  • PPP1INL
  • Protein kinase C-potentiated inhibitor protein of 17 kDa
  • protein phosphatase 1 regulatory subunit 14A
  • protein phosphatase 1, regulatory (inhibitor) subunit 14A

Background

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TNF-alpha
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Applications: ELISA
CPI17 alpha
Antibody (4H10) - BSA
NBP1-04336
CPI17 alpha Antibody (4H10) - BSA Free
Species: Hu
Applications: WB, ELISA, ICC/IF

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Bioinformatics

Gene Symbol PPP1R14A
Uniprot