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Rat Connexin 43/GJA1 ELISA Kit (Colorimetric)

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ELISA: Rat Connexin 43/GJA1 ELISA Kit (Colorimetric) [NBP3-06835] - Samples were spiked with high concentrations of Rat Connexin 43/GJA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Connexin 43/GJA1 ELISA Kit (Colorimetric) [NBP3-06835] - Samples were spiked with high concentrations of Rat Connexin 43/GJA1 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Rat Connexin 43/GJA1 ELISA Kit (Colorimetric) [NBP3-06835] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

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Rat Connexin 43/GJA1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Connexin 43/GJA1 in samples. No significant cross-reactivity or interference between Rat Connexin 43/GJA1 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.84
Intra-Assay
4.93
Spike Recovery
87 - 104 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
GJA1

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Connexin 43/GJA1 ELISA Kit (Colorimetric)

  • Connexin 43
  • connexin-43
  • CX43
  • CX43gap junction protein, alpha-like
  • DFNB38
  • Gap junction 43 kDa heart protein
  • gap junction alpha-1 protein
  • gap junction protein, alpha 1, 43kDa (connexin 43)
  • gap junction protein, alpha 1, 43kDa
  • GJA1
  • GJAL
  • HSS
  • ODD
  • ODDD
  • ODOD
  • SDTY3

Background

Connexin 43/GJA1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major cardiac gap junction protein, which is thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Connexin: Bridging the Gap of Intercellular Communication
Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a...  Read full blog post.
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Contact Information

Product PDFs

  SDS
  Rat Connexin 43/GJA1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol GJA1