![Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - GJB4 monoclonal antibody (M01), clone 1E3-1C12 Analysis of GJB4 expression in Hela.](http://images.novusbio.com/fullsize/Connexin-30-3-GJB4-Antibody-1E3-1C12-Western-Blot-H00127534-M01-img0005.jpg "Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - GJB4 monoclonal antibody (M01), clone 1E3-1C12 Analysis of GJB4 expression in Hela.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 1E3-1C12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | GJB4 (AAH34709, 1 a.a. ~ 266 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNWAFLQGLLSGVNKYSTVLSRIWLSVVFIFRVLVYVVAAEEVWDDEQKDFVCNTKQPGCPNVCYDEFFPVSHVRLWALQLILVTCPSLLVVMHVAYREERERKHHLKHGPNAPSLYDNLSKKRGGLWWTYLLSLIFKAAVDAGFLYIFHRLYKDYDMPRVVACSVEPCPHTVDCYISRPTEKKVFTYFMVTTAAICILLNLSEVFYLVGKRCMEIFGPRHRRPRCRECLPDTCPPYVLSQGGHPEDGNSVLMKAGSAPVDAGGYP |
| Specificity | GJB4 - gap junction protein, beta 4 (connexin 30.3) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GJB4 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - Analysis of GJB4 expression in transfected 293T cell line by GJB4 monoclonal antibody (M01), clone 1E3-1C12.Lane 1: GJB4 transfected lysate(30 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Connexin-30-3-GJB4-Antibody-1E3-1C12-Western-Blot-H00127534-M01-img0006.jpg "Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - Analysis of GJB4 expression in transfected 293T cell line by GJB4 monoclonal antibody (M01), clone 1E3-1C12.Lane 1: GJB4 transfected lysate(30 KDa).Lane 2: Non-transfected lysate.")
![ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - Detection limit for recombinant GST tagged GJB4 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Connexin-30-3-GJB4-Antibody-1E3-1C12-ELISA-H00127534-M01-img0007.jpg "ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - Detection limit for recombinant GST tagged GJB4 is approximately 3ng/ml as a capture antibody.")
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