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Human Complement C5 ELISA Kit (Colorimetric)

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ELISA: Human Complement C5 ELISA Kit (Colorimetric) [NBP2-60620] - Representative standard curve.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell Culture, Saliva, Milk, CSF
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.1 ng/mL (example only; lot dependent)

Order Details

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Human Complement C5 ELISA Kit (Colorimetric) Summary

Description
This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.1 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 9.90 (example only; lot dependent)
Intra-Assay
%CV < 3.10 (example only; lot dependent)
Spike Recovery
101% (example only; lot dependent)
Kit Type
ELISA Kit (Colorimetric)
Gene
C5

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Alternate Names for Human Complement C5 ELISA Kit (Colorimetric)

  • anaphylatoxin C5a analog
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
  • C5
  • complement C5
  • complement component 5
  • CPAMD4MGC142298
  • FLJ17816
  • FLJ17822

Background

C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kDa) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Complement C5 ELISA Kit (NBP2-60620). (Showing 1 - 1 of 1 FAQ).

  1. NBP2-60620 can this product be used to deteremin C5a?
    • This Human Complement C5 ELISA kit is specifically designed to detect the uncleaved C5. There is no guarantee that it can pick up the signal from either C5a or C5b once C5 is cleaved.

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Research Areas for Complement C5 ELISA Kit (NBP2-60620)

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Blogs on Complement C5.

Complement C3 - The Most Important Protein in the Complement System
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

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Bioinformatics

Gene Symbol C5