Human Complement C5 ELISA Kit (Colorimetric) Summary
Description |
This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples. |
Standard Curve Range |
0.156 - 10 ng/mL (example only; lot dependent) |
Sensitivity |
0.1 ng/mL (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 9.90 (example only; lot dependent) |
Intra-Assay |
%CV < 3.10 (example only; lot dependent) |
Spike Recovery |
101% (example only; lot dependent) |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
C5 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage is content dependent. |
Alternate Names for Human Complement C5 ELISA Kit (Colorimetric)
Background
C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kDa) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Complement C5 ELISA Kit (NBP2-60620). (Showing 1 - 1 of 1 FAQ).
-
NBP2-60620 can this product be used to deteremin C5a?
- This Human Complement C5 ELISA kit is specifically designed to detect the uncleaved C5. There is no guarantee that it can pick up the signal from either C5a or C5b once C5 is cleaved.