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Collectrin/TMEM27 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Collectrin/TMEM27 Antibody [NBP2-13450] - Immunofluorescent staining of human cell line HaCaT shows localization to endoplasmic reticulum & vesicles.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450] - Staining in human kidney and lymph node tissues using anti-TMEM27 antibody. Corresponding TMEM27 RNA-seq data are ...read more
Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: Collectrin/TMEM27 Antibody [NBP2-13450] - Staining of human lymph node shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Collectrin/TMEM27 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: LVTAIHAELCQPGAENAFKVRLSIRTALGDKAYAWDTNEEYLFKAMVAFSMRKVPNREATEISHVLLCNVTQRVSFWFVVTDPSKNHTLPAVEVQSAIRMNKNRINNAFFLNDQTLE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLTRN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Collectrin/TMEM27 Protein (NBP2-13450PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Collectrin/TMEM27 Antibody

  • Collectrin
  • kidney-specific membrane protein
  • NX17
  • NX-17
  • TMEM27
  • transmembrane protein 27NX170610008J07Rik

Background

The Tmem27 gene encodes a transmembrane protein that is important for trafficking amino acid transporters to the apical brushborder of proximal tubules. It also plays a role in controlling insulin exocytosis by regulating formation of theSNARE (soluble N-ethylm

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CLTRN