Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence) Summary
Specificity |
This kit recognizes Human COL6a1 in samples. No significant cross-reactivity or interference between Human COL6a1 and analogues was observed. |
Standard Curve Range |
31.25-2000 pg/mL |
Sensitivity |
18.75 pg/mL |
Assay Type |
Sandwich-CLIA |
Inter-Assay |
CV% < 8.27% |
Intra-Assay |
CV% < 8.78% |
Spike Recovery |
86-113% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Chemiluminescence) |
Gene |
COL6A1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)
Background
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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