![Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23231_rabbit-cofilin-p-ser3-pab-199202312403712.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).")
| Reactivity | Hu, Mu, Rt, ChSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | A synthetic phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 3 in human Cofilin. This sequence has 100% homology with similar regions of rat and mouse Cofilin, and has two amino acid differences from human Cofilin 2. [UniProt# P23528] |
| Modification | p Ser3 |
| Specificity | This antibody was cross-adsorbed to unphosphorylated Coflin peptide then affinity purified using phospho-Cofilin (Ser-3) peptide (without carrier). The antibody detects a 19 kDa protein corresponding to the molecular mass of phosphorylated Cofilin on SDS-PAGE immunoblots of Jurkat cells. This band can be removed by lambda phosphatase treatment. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CFL1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cofilin Antibody (NBP3-23231)Find related products by research area.
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| Gene Symbol | CFL1 |
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