![Western Blot: Cofilin Antibody (PSH0-33) [NBP3-32204] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF-7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: A431 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (30 ug/Lane) Lane 11: VERO cell lysate (30 ug/Lane) Lane 12: NIH/3T3 cell lysate (22 ug/Lane) Lane 13: PC-12 cell lysate (30 ug/Lane) Lane 14: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 20 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32204) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-27820241631651.jpg "Western Blot: Cofilin Antibody (PSH0-33) [NBP3-32204] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF-7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: A431 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (30 ug/Lane) Lane 11: VERO cell lysate (30 ug/Lane) Lane 12: NIH/3T3 cell lysate (22 ug/Lane) Lane 13: PC-12 cell lysate (30 ug/Lane) Lane 14: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 20 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32204) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | PSH0-33 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within human Cofilin aa 117-166 / 166. (Uniprot: P23528) |
| Localization | Nucleus matrix, Cytoplasm, Cytoskeleton, Cell projection. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CFL1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cofilin Antibody (NBP3-32204)Find related products by research area.
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| Gene Symbol | CFL1 |
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH0-33) [NBP3-32204] - Immunocytochemistry analysis of NIH/3T3 cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-29202410334062.jpg "Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH0-33) [NBP3-32204] - Immunocytochemistry analysis of NIH/3T3 cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: Cofilin Antibody (PSH0-33) [NBP3-32204] - Flow cytometric analysis of Hela cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32204, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-29202416372129.jpg "Flow Cytometry: Cofilin Antibody (PSH0-33) [NBP3-32204] - Flow cytometric analysis of Hela cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32204, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")