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Canine Clusterin/APOJ ELISA Kit (Colorimetric)

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ELISA: Canine Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-59978] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Canine Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-59978] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Canine Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-59978] - Standard Curve Reference

Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
7.813 ng/mL - 500 ng/mL
Sensitivity
4.963 ng/mL

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Canine Clusterin/APOJ ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of dog clusterin. No significant cross-reactivity or interference between dog clusterin and analogues was observed.
Standard Curve Range
7.813 ng/mL - 500 ng/mL
Sensitivity
4.963 ng/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CLU

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. HRP-conjugate (100 x concentrate)
  4. HRP-conjugate Diluent
  5. Instruction manual
  6. Sample Diluent
  7. Standard (Freeze dried)
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (25 x concentrate)

Alternate Names for Canine Clusterin/APOJ ELISA Kit (Colorimetric)

  • 40
  • 40, sulfated glycoprotein 2
  • Aging-associated gene 4 protein
  • aging-associated protein 4
  • APOJ
  • apo-J
  • Apolipoprotein J
  • CLI
  • CLIclusterin (complement lysis inhibitor, SP-40
  • CLU
  • Clusterin
  • Complement cytolysis inhibitor
  • complement lysis inhibitor
  • Complement-associated protein SP-40
  • Ku70-binding protein 1
  • KUB1SGP2
  • MGC24903
  • NA1/NA2
  • SGP-2
  • SP-40
  • sulfated glycoprotein 2
  • Testosterone-repressed prostate message 2
  • testosterone-repressed prostate message 2, apolipoprotein J)
  • TRPM-2
  • TRPM-2TRPM2

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with clusterin. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for clusterin. The competitive inhibition reaction is launched between with pre-coated clusterin and clusterin in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of clusterin in the samples. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CLU