![Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody [NBP1-06027] - Immunofluorescence analysis of paraformaldehyde fixed PC12 suspension cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-06027_goat-polyclonal-clusterin-apoj-antibody-16520239183316.jpg "Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody [NBP1-06027] - Immunofluorescence analysis of paraformaldehyde fixed PC12 suspension cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-EKALQEYRRKSRAE corresponding to C-Terminus according to NP_038520.1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CLU |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 38kDa band observed in Mouse and Rat Brain lysates.(calculated MW of 51.7kDa according to Mouse NP_038520.2 and 51.4kDa according to Rat XP_006252156.1). This molecular weight is routinely observed by other sources and thought to correspond to the alpha chain of APOJ. Primary incubation 1 hour at room temperature. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CLU |
| Entrez | |
| Uniprot |
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![Western Blot: Clusterin/APOJ Antibody [NBP1-06027] - (0.1ug/ml) staining of Mouse (A) and (0.3ug/ml) Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-06027_goat-polyclonal-clusterin-apoj-antibody-1652023918330.jpg "Western Blot: Clusterin/APOJ Antibody [NBP1-06027] - (0.1ug/ml) staining of Mouse (A) and (0.3ug/ml) Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
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