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CLNS1A Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CLNS1A Antibody [NBP2-33958] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CLNS1A Antibody [NBP2-33958] - Staining of human duodenum shows strong cytoplasmic and nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CLNS1A Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: YTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDSIRDYEDGMEVDTTPTVAGQFEDADVDH
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLNS1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot Reported in scientific literature (PMID:34358446)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CLNS1A Protein (NBP2-33958PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-33958 in the following applications:

Publications
Read Publication using
NBP2-33958 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CLNS1A Antibody

  • chloride channel regulatory protein
  • Chloride channel, nucleotide sensitive 1A
  • chloride channel, nucleotide-sensitive, 1A
  • Chloride conductance regulatory protein ICln
  • Chloride ion current inducer protein
  • ClCI
  • CLNS1B
  • i(Cln)
  • ICln
  • methylosome subunit pICln
  • Reticulocyte pICln
  • reticulocyte protein ICln

Background

The CLNS1A gene encodes a protein that functions in multiple regulatory pathways. The encoded protein complexes with numerouscytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoproteinbiosynthesis, platelet activati

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CLNS1A Antibody (NBP2-33958)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Review for CLNS1A Antibody (NBP2-33958) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-33958:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot CLNS1A NBP2-33958
Enlarge
5
reviewed by:
Kathleen Mulvaney
WB Human 03/20/2019
View

Summary

ApplicationWestern Blot
Sample TestedHuman pancreatic cancer cell lines MiaPaCa-2, AsPC-1, and BxPC-3
SpeciesHuman
LotR31249

Comments

CommentsMiaPaca2 cells stably expressing Cas9 were infected with AAVS1 control or CLNS1A/pICLn targeting guide RNA lentivirus for 48h, selected for guide RNA infection with 1ug/ml puromycin for an additional 48h. Cells were lysed in RIPA, cleared by centrifugation, soluble protein was quantified by BCA, and 20ug of whole cell extract was added in each lane. CLNS1A/pICLn was detected with this antibody at 1:2,000 dilution overnight at 4C followed by secondary antibody 1:5,000 (LiCor) for 30 minutes at room temp and developed on a LiCor imager.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CLNS1A Antibody (NBP2-33958) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on CLNS1A

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Kathleen Mulvaney
03/20/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol CLNS1A
Uniprot