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Clathrin light chain Antibody (SPM174)

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Immunohistochemistry-Paraffin: Clathrin light chain Antibody (SPM174) [NBP2-53232] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Clathrin, LC Monoclonal Antibody (SPM174).
Immunohistochemistry-Paraffin: Clathrin light chain Antibody (SPM174) [NBP2-53232] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Clathrin, LC Monoclonal Antibody (SPM174).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
SPM174
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54435AF350
Alexa Fluor 405
NBP2-54435AF405
Alexa Fluor 488
NBP2-54435AF488
Alexa Fluor 532
NBP2-54435AF532
Alexa Fluor 594
NBP2-54435AF594
Alexa Fluor 647
NBP2-54435AF647
Alexa Fluor 700
NBP2-54435AF700
Alexa Fluor 750
NBP2-54435AF750
Allophycocyanin
NBP2-54435APC
Biotin
NBP2-54435B
CoraFluor 1
NBP2-54435CL1
DyLight 350
NBP2-54435UV
DyLight 405
NBP2-54435V
DyLight 488
NBP2-54435G
DyLight 550
NBP2-54435R
DyLight 594
NBP2-54435DL594
DyLight 650
NBP2-54435C
DyLight 680
NBP2-54435FR
DyLight 755
NBP2-54435IR
FITC
NBP2-54435F
HRP
NBP2-54435H
Janelia Fluor 525
NBP2-54435JF525
Janelia Fluor 549
NBP2-54435JF549
Janelia Fluor 585
NBP2-54435JF585
Janelia Fluor 635
NBP2-54435JF635
Janelia Fluor 646
NBP2-54435JF646
Janelia Fluor 669
NBP2-54435JF669
PE
NBP2-54435PE
PerCP
NBP2-54435PCP
mFluor Violet 450 SE
NBP2-54435MFV450
mFluor Violet 500 SE
NBP2-54435MFV500
mFluor Violet 610 SE
NBP2-54435MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-54435-100ug
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Clathrin light chain Antibody (SPM174) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54435)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Purified recombinant N-terminal fragment of human Clathrin Light Chain
Localization
Cytoplasmic
Specificity
Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CLTA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Clathrin light chain Antibody (SPM174)

  • clathrin light chain A
  • clathrin, light chain A
  • Lca
  • light polypeptide (Lca)

Background

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Transcript Variant: This variant (1) lacks alternate in-frame exons, compared to variant 2, resulting in a shorter protein (isoform a), compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLTA
Uniprot