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Chorionic Gonadotropin alpha Chain (hCG alpha) Overexpression Lysate

Name: Chorionic Gonadotropin alpha Chain (hCG alpha) Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-09130
Price: 679 USD

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Western Blot: Glycoprotein Hormones, alpha subunit Overexpression Lysate (Adult Normal) [NBL1-09130] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycoprotein ...read more

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Summary

Reactivity	HuSpecies Glossary
Applications	WB

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Chorionic Gonadotropin alpha Chain (hCG alpha) Overexpression Lysate Summary

Description

Chorionic Gonadotropin alpha Chain (hCG alpha) Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC203146

Accession#: NM_000735

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Gene

CGA

Applications/Dilutions

Dilutions	Western Blot
Application Notes	This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW	10.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	RIPA buffer

Lysate Details for Array

Type

Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Chorionic Gonadotropin alpha Chain (hCG alpha) Overexpression Lysate

alpha HCG
CGA glycoprotein hormones, alpha polypeptide
CGA
CG-ALPHA
Chorionic Gonadotropin alpha Chain
FSHA
GPHa
GPHa1
HCG
LHA
TSHA

Background

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Chorionic Gonadotropin alpha Chain (hCG alpha) Lysate (NBL1-09130) (0)

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Bioinformatics

Gene Symbol

CGA

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Chorionic Gonadotropin alpha Chain (hCG alpha) Overexpression Lysate

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Chorionic Gonadotropin alpha Chain (hCG alpha) Overexpression Lysate Summary

Chorionic Gonadotropin alpha Chain (hCG alpha) Transient Overexpression Lysate

Applications/Dilutions

Packaging, Storage & Formulations

Lysate Details for Array

Notes

Alternate Names for Chorionic Gonadotropin alpha Chain (hCG alpha) Overexpression Lysate

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Chorionic Gonadotropin alpha Chain (hCG alpha) Lysate (NBL1-09130) (0)

Reviews for Chorionic Gonadotropin alpha Chain (hCG alpha) Lysate (NBL1-09130) (0)

Product General Protocols

Video Protocols

FAQs for Chorionic Gonadotropin alpha Chain (hCG alpha) Lysate (NBL1-09130) (0)

Additional Chorionic Gonadotropin alpha Chain (hCG alpha) Products

Research Areas for Chorionic Gonadotropin alpha Chain (hCG alpha) Lysate (NBL1-09130)

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Bioinformatics