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Human Chordin-like 2/CHRDL2 ELISA Kit (Colorimetric)

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ELISA: Human Chordin-like 2/CHRDL2 ELISA Kit (Colorimetric) [NBP3-27378] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.058 ng/mL (example only; lot dependent)

Order Details

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Human Chordin-like 2/CHRDL2 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.058 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CHRDL2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Chordin-like 2/CHRDL2 ELISA Kit (Colorimetric)

  • BNF-1
  • Chordin like 2
  • Chordin-like 2
  • chordin-like protein 2
  • CHRDL2

Background

BNF-1, also known as Chordin-Like 2, (CHRDL2), is a glycoprotein that negatively regulates cartilage formation by binding Activin A and BMPs and preventing interaction with their receptors. BNF1 contains three von Willebrand factor type C domains. Alternate splicing of mouse BNF1 generates intracellular as well as secreted variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol CHRDL2