![Western Blot: CHD3 Antibody [NB100-60412] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD3 antibody NB100-60412 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CHD3 was also immunoprecipitated by previous lot 1 of this antibody and by rabbit anti-CHD3 antibody NB100-60413, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/CHD3-Antibody-Western-Blot-NB100-60412-img0005.jpg "Western Blot: CHD3 Antibody [NB100-60412] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD3 antibody NB100-60412 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CHD3 was also immunoprecipitated by previous lot 1 of this antibody and by rabbit anti-CHD3 antibody NB100-60413, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Please note, this product recently (April 19, 2012) changed concentrations from 0.2 mg/ml to 1.0 mg/ml. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1575 and 1625 of human chromodomain helicase DNA binding protein 3 using the numbering given in entry NP_001005273.1 (GeneID 1107). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CHD3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Theoretical MW | 226 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CHD3 Antibody (NB100-60412)Find related products by research area.
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![Immunohistochemistry-Paraffin: CHD3 Antibody [NB100-60412] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CHD3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CHD3-Antibody-Immunohistochemistry-Paraffin-NB100-60412-img0007.jpg "Immunohistochemistry-Paraffin: CHD3 Antibody [NB100-60412] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CHD3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")