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Cdc6 [p Ser54] Antibody (SR2085)

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Western Blot: Cdc6 [p Ser 54] Antibody (SR2085) [NBP3-21570] - Western blot analysis using NBP3-21570 in (1) Raji cell lysate; (2) Raji + FBS cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
SR2085
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Cdc6 [p Ser54] Antibody (SR2085) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human phospho-Cdc6 [Ser54] (Uniprot #: Q99741)
Modification
p Ser54
Specificity
Detects endogenous levels of total phospho-Cdc6 [Ser54]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CDC6
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Cdc6 [p Ser54] Antibody (SR2085)

  • CDC18LHsCDC18
  • Cdc18-related protein
  • CDC6 (cell division cycle 6, S. cerevisiae) homolog
  • CDC6 cell division cycle 6 homolog (S. cerevisiae)
  • CDC6 cell division cycle 6 homolog
  • CDC6-related protein
  • cell division control protein 6 homolog
  • cell division cycle 6 homolog (S. cerevisiae)
  • cell division cycle 6 protein
  • HsCdc18
  • HsCDC6
  • p62(cdc6)

Background

Cdc6 is encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cdc6 Antibody (NBP3-21570) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC6