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CDC2/CDK1 Antibody (1A4-1A9)

Images

 
Western Blot: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9.Lane 1: CDC2 transfected lysate(34.1 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of monoclonal antibody to CDC2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 0.3 ug/ml.
Western Blot: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - CDC2 monoclonal antibody (M01), clone 1A4-1A9 Analysis of CDC2 expression in Hela.
ELISA: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Detection limit for recombinant GST tagged CDC2 is approximately 30ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1A4-1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CDC2/CDK1 Antibody (1A4-1A9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CDC2 (AAH14563, 1 a.a. ~ 297 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM
Specificity
CDC2 - cell division cycle 2, G1 to S and G2 to M
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDK1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CDC2/CDK1 Antibody (1A4-1A9)

  • CDC2
  • CDC28A
  • CDC2MGC111195
  • CDK1
  • cell cycle controller CDC2
  • Cell division control protein 2 homolog
  • Cell division protein kinase 1
  • cyclin-dependent kinase 1
  • DKFZp686L20222
  • EC 2.7.11.22
  • EC 2.7.11.23
  • G1 to S and G2 to M
  • p34 protein kinase
  • P34CDC2

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CDK1
Entrez
OMIM
Uniprot