Home » CD99 » CD99 ELISA Kits » Human CD99 ELISA Kit (Colorimetric)
Novus Biologicals products are now on bio-techne.com

Human CD99 ELISA Kit (Colorimetric)

 Submit a Review

Images

 
(
Enlarge)
ELISA: Human CD99 ELISA Kit (Colorimetric) [NBP2-75166] - Samples were spiked with high concentrations of Human CD99 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human CD99 ELISA Kit (Colorimetric) [NBP2-75166] - Samples were spiked with high concentrations of Human CD99 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human CD99 ELISA Kit (Colorimetric) [NBP2-75166] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Human CD99 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CD99 in samples. No significant cross-reactivity or interference between Human CD99 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.52%
Intra-Assay
CV% < 5.7%
Spike Recovery
86-104%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CD99

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human CD99 ELISA Kit (Colorimetric)

  • 12E7
  • CD99 antigenY homolog
  • CD99 molecule
  • CD99
  • E2 antigen
  • HBA71
  • MIC2 (monoclonal 12E7)
  • MIC2
  • MIC2Y
  • MSK5X
  • pilr-1
  • PILR-L
  • Protein MIC2
  • surface antigen MIC2
  • T-cell surface glycoprotein E2

Background

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
Vimentin Antibody
NB300-223
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
Immunohistochemistry-Frozen Vimentin Antibody
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
(6 Reviews)
(70 Publications)
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
CD34 Antibody
[Unconjugated]
AF4117
Immunohistochemistry CD34 Antibody [Unconjugated]
(3 Reviews)
(37 Publications)
Species: Rt
Applications: IHC, WB
EWSR1 Antibody
NB200-182
Immunohistochemistry-Paraffin EWSR1 Antibody
Immunoprecipitation EWSR1 Antibody
Western Blot EWSR1 Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, Simple Western, WB
Desmin Antibody
[Unconjugated]
AF3844
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Desmin Antibody [Unconjugated]
(2 Reviews)
(34 Publications)
Species: Hu, Mu
Applications: IHC
Enolase
2/Neuron-specific Eno ...
NB110-58870
Immunocytochemistry/ Immunofluorescence Enolase 2/Neuron-specific Enolase Antibody
Western Blot Enolase 2/Neuron-specific Enolase Antibody
Simple Western Enolase 2/Neuron-specific Enolase Antibody
(2 Reviews)
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KD, Simple Western, WB
Bcl-2 Antibody
NB100-56098
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
(2 Reviews)
(26 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Synaptophysin
Antibody
NB300-653
Immunohistochemistry-Frozen Synaptophysin Antibody
Western Blot Synaptophysin Antibody
Immunohistochemistry-Paraffin Synaptophysin Antibody
(2 Reviews)
(11 Publications)
Species: Ch, Fe, Hu, Pm, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
MUC1 Antibody
(SM3) - BSA Free
NB120-22711
Immunocytochemistry/ Immunofluorescence MUC1 Antibody (SM3) - BSA Free
Flow Cytometry MUC1 Antibody (SM3) - BSA Free
Immunocytochemistry/ Immunofluorescence MUC1 Antibody (SM3) - BSA Free
(14 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NCAM-1/CD56
Antibody [Unconjugate
AF2408
Western Blot NCAM-1/CD56 Antibody [Unconjugated]
Simple Western NCAM-1/CD56 Antibody [Unconjugated]
Immunocytochemistry NCAM-1/CD56 Antibody [Unconjugated]
(24 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, KO, Simple Western, WB
CD117/c-kit
Antibody [Unconjugate
AF1356
Simple Western CD117/c-kit Antibody [Unconjugated]
Western Blot CD117/c-kit Antibody [Unconjugated]
Immunohistochemistry CD117/c-kit Antibody [Unconjugated]
(4 Reviews)
(116 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Simple Western, WB
ETFA Antibody
NBP1-84854
Immunocytochemistry/ Immunofluorescence ETFA Antibody
Immunohistochemistry-Paraffin ETFA Antibody
Immunohistochemistry-Paraffin ETFA Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
Cytokeratin 20
Antibody (SA35-03)
NBP2-67309
Flow Cytometry Cytokeratin 20 Antibody (SA35-03)
Immunocytochemistry/ Immunofluorescence Cytokeratin 20 Antibody (SA35-03)
Immunohistochemistry-Paraffin Cytokeratin 20 Antibody (SA35-03)
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
CD45 Antibody
[Unconjugated]
AF114
Immunohistochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CD45 Antibody [Unconjugated]
(1 Review)
(70 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
S100B Antibody
NBP1-87102
Immunocytochemistry/ Immunofluorescence S100B Antibody
Immunohistochemistry-Paraffin S100B Antibody
Immunohistochemistry-Paraffin S100B Antibody
(14 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
S100A1 Antibody
NBP1-87103
Western Blot S100A1 Antibody
Immunohistochemistry-Paraffin S100A1 Antibody
Immunohistochemistry-Paraffin S100A1 Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Neprilysin/CD10
Antibody [Unconjugate
AF1126
Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]
Western Blot Neprilysin/CD10 Antibody [Unconjugated]
Western Blot Neprilysin/CD10 Antibody [Unconjugated]
(22 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
TdT Antibody
NBP2-31368
Western Blot TdT Antibody
Immunohistochemistry-Paraffin TdT Antibody
Immunohistochemistry-Paraffin TdT Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for CD99 ELISA Kit (NBP2-75166) (0)

There are no publications for CD99 ELISA Kit (NBP2-75166).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD99 ELISA Kit (NBP2-75166) (0)

There are no reviews for CD99 ELISA Kit (NBP2-75166). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD99 ELISA Kit (NBP2-75166) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CD99 Products

Research Areas for CD99 ELISA Kit (NBP2-75166)

Find related products by research area.

Blogs on CD99

There are no specific blogs for CD99, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2&nbsp;mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165&nbsp;kDa (as indicated) using 5&nbsp;µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&nbsp;# HAF109). This experiment was conducted under reducing&nbsp;conditions and using the 12-230 kDa separation system.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
CD117/c-kit Antibody [Unconjugated]
AF1356
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Vimentin Antibody
NB300-223

Contact Information

Product PDFs

  SDS
  Human CD99 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human CD99 ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol CD99