![Immunocytochemistry/Immunofluorescence: CD81 Antibody (1D6) [NBP2-81096] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 1D6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81096, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Immunocytochemistry-Immunofluorescence-NBP2-81096-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD81 Antibody (1D6) [NBP2-81096] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 1D6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81096, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.")
| Reactivity | Hu, Gt, Pm, ShSpecies Glossary |
| Applications | WB, ELISA, Flow, Func, ICC/IF, IHC, IP |
| Clone | 1D6 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was produced by immunizing mice with 5A6-aggregated OCI-LY8 B cells. |
| Specificity | This antibody is specific for human CD81, which is expressed on most tissues with the exception of red blood cells and platelets. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CD81 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody is a strong inducer of homotypic adhesion in hematolymphoid cells (Levy, 1998). This antibody also has an anti-proliferative effect, and is able to induce growth inhibition of B cell lines. Triggering of CD81 by this antibody induces a significant upregulation of the constituitive release of TNF-alpha by JY cells, an Eptsein-Barr virus-positive B lymphoblastoid cell line (Altomonte, 1995). |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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CD19: An Undoubted Biomarker for B Cells CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t... Read full blog post. |
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CD81/TAPA1: I'm on Tapa the Cell Target of the antiproliferative 1 (TAPA1), also known as CD81, is found in the plasma membrane in lymphocytes and plays an important role in the regulation of lymphoma cell growth. This transmembrane 4 superfamily (TM4SF) protein is primarily found on... Read full blog post. |
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| Gene Symbol | CD81 |
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