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CD7 Antibody (4D4F8) - BSA Free

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Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis of lung cancer tissues using CD7 mouse mAb with DAB staining.
Flow Cytometry: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis of Jurkat cells using CD7 mouse mAb (green) and negative control (red).
Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against human CD7 recombinant protein. (Expected MW is 36.6 kDa).
ELISA: CD7 Antibody (4D4F8) [NBP2-37367] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
4D4F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37367-0.025ml
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CD7 Antibody (4D4F8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD7 (AA: 72-175) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD7
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD7 Antibody (4D4F8) - BSA Free

  • CD7 antigen (p41)
  • CD7 antigen
  • CD7 molecule
  • CD7
  • GP40T-cell surface antigen Leu-9
  • LEU-9
  • T-cell antigen CD7
  • T-cell leukemia antigen
  • Tp40
  • TP41
  • TP41p41 protein

Background

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD7 Antibody (NBP2-37367) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD7