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CD39L3/ENTPD3 Antibody [Unconjugated]

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Simple Western lane view shows lysates of Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD39L3/ENTPD3 at approximately 127 kDa (as indicated) using 20 µg/mL of Sheep Anti-Mouse CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4464) . This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Simple Western lane view shows lysates of Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD39L3/ENTPD3 at approximately 127 kDa (as indicated) using 20 µg/mL of ...read more
Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, mouse salivary gland, and mouse spinal cord. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse CD39L3/ENTPD3 Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4464
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4464-SP
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CD39L3/ENTPD3 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L3/ENTPD3
Gln44-Pro485
Accession # AAH79871
Specificity
Detects mouse CD39L3/ENTPD3 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human CD39L3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ENTPD3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF4464 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39L3/ENTPD3 Antibody [Unconjugated]

  • ATPDase
  • CD39 antigen-like 3
  • CD39L3
  • CD39L3EC 3.6.1.5
  • CD39-like 3
  • EC 3.6.1
  • Ecto-apyrase 3
  • Ecto-ATP diphosphohydrolase 3
  • Ecto-ATPase 3
  • Ecto-ATPDase 3
  • ectonucleoside triphosphate diphosphohydrolase 3
  • ENTPD3
  • FLJ93839
  • HB6
  • HB6NTPDase 3
  • NTPDase-3

Background

CD39L3 (CD39-like 3; also HB6 and ectonucleoside triphosphate diphosphohydrolase-3/eNTPDase 3) is an 80 kDa, class I member of the GDA1/CD39 NTPase family of transmembrane (TM) enzymes. It is a cell surface glycoprotein that hydrolyses both extracellular ATP and ADP. Thus, it controls ATP activation of P2X ion channel purinoreceptors. Mouse CD39L3 is 529 amino acids (aa) in length. It contains two TM segments plus an N- and C-terminal cytoplasmic region. Based on human CD39L3, the mouse extracellular domain (ECD) will contain aa 44-485. Within the ECD, mouse CD39L3 shares 82% and 94% aa identity with human and rat CD39L3 ECD, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD39L3/ENTPD3 Antibody (AF4464)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol ENTPD3
Uniprot