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CD21 Antibody (CL14556)

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Western blot analysis in human cell line Daudi.
Western blot analysis in human cell line Daudi.
Staining of human lymph node shows strong membranous positivity in germinal center.
Staining of human tonsil shows strong membranous positivity in germinal center.
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Staining of human small intestine shows strong membranous positivity in lymph node.
Orthogonal Strategies: Analysis in human tonsil and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL14556
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
       

Orthogonal Strategies

  

Order Details

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CD21 Antibody (CL14556) Summary

Immunogen
This antibody was developed against a Synthetic Peptide of P20023. Actual immunogen sequence is proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CR2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD21 Antibody (CL14556)

  • C3DR
  • C3DRSLEB9
  • CD21 antigen
  • CD21
  • Complement C3d receptor
  • complement component (3d/Epstein Barr virus) receptor 2
  • complement receptor type 2
  • CR2
  • EBV receptor
  • Epstein-Barr virus receptor

Background

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CR2