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CBL Antibody

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Western Blot: CBL Antibody [NBP2-15730] - A. 30 ug THP-1 whole cell lysate/extract 7.5 % SDS-PAGE CBL antibody [C2C3], C-term dilution: 1:1000
Western Blot: CBL Antibody [NBP2-15730] - A. 30 ug THP-1 whole cell lysate/extract 7.5 % SDS-PAGE CBL antibody [C2C3], C-term dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: CBL Antibody [NBP2-15730] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:50 dilution.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CBL Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human CBL. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBL
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:3000
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 40% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for CBL Antibody

  • Cas-Br-M (murine) ecotropic retroviral transforming sequence
  • Casitas B-lineage lymphoma proto-oncogene
  • CBL
  • CBL2
  • CBL2E3 ubiquitin-protein ligase CBL
  • EC 6.3.2
  • EC 6.3.2.-
  • oncogene CBL2
  • Proto-oncogene c-Cbl
  • RING finger protein 55
  • RNF55
  • RNF55C-CBL
  • Signal transduction protein CBL

Background

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CBL
Uniprot