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Caspase-7 Antibody (10-1-62)

Images

 
Western Blot: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Analysis of human Jurkat and Jurkat induced cell lysates probed with AAM-137
Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Human spleen tissue stained with Caspase-7, mAb (10-1-62) at 10ug/ml.
Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Human colon tissue stained with Caspase-7, mAb (10-1-62) at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC
Clone
10-1-62
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Caspase-7 Antibody (10-1-62) Summary

Immunogen
Recombinant human Caspase-7 protein (full length)
Specificity
Caspase-7 (10-1-62)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CASP7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
Control
Jurkat Staurosporine Treated / Untreated Cell Lysate

Reactivity Notes

May react with Hamster. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 10mM sodium phosphate, 50mM sodium chloride, and 0.1mM PMSF
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-7 Antibody (10-1-62)

  • apoptosis-related cysteine protease
  • Apoptotic protease Mch-3
  • CASP7
  • CASP-7
  • caspase 7, apoptosis-related cysteine peptidase
  • Caspase7
  • Caspase-7
  • EC 3.4.22
  • EC 3.4.22.60
  • ICE-like apoptotic protease 3
  • Lice2 alpha/beta/gamma
  • Mch3

Background

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-7 Antibody (NBP1-19230) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Caspase-7 Products

Research Areas for Caspase-7 Antibody (NBP1-19230)

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Blogs on Caspase-7.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP7
Entrez