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Images
Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 μM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase‑3 Alexa Fluor® ...read more
Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 μM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase‑3 Alexa Fluor® ...read more
12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Caspase-3 Antibody (269518) [Alexa Fluor® 488]
Apopain
apoptosis-related cysteine protease
CASP3
CASP-3
caspase 3, apoptosis-related cysteine peptidase
Caspase3
Caspase-3
CPP32
CPP-32
CPP32B
CPP32SREBP cleavage activity 1
Cysteine protease CPP32
EC 3.4.22
EC 3.4.22.56
LICE-1
PARP cleavage protease
procaspase3
Protein Yama
SCA-1
YAMA
Background
Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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