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Caspase-3 Antibody (269518) [Alexa Fluor® 488]

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Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 μM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase‑3 Alexa Fluor® ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
269518
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 488
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Caspase-3 Antibody (269518) [Alexa Fluor® 488] Summary

Immunogen
KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # U26943
Specificity
Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CASP3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells
Publications
Read Publications using
IC835G in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody (269518) [Alexa Fluor® 488]

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CASP3
Uniprot